| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the Department of Anatomy II, University of Erlangen-Nürnberg, Erlangen, Germany; the 2 Laboratory of Mechanisms of Ocular Diseases, National Institutes of Health, National Eye Institute, Bethesda, Maryland; and the 3 Eye Hospital of the University of Munich, Munich, Germany.
PURPOSE. To study distribution and cellular localization of myocilin/trabecular meshwork–inducible glucocorticoid response protein (TIGR) in the human eye.
METHODS. A peptide antibody against a portion of the myosin-like domain of myocilin/TIGR was developed. Different ocular tissues from three human donors were investigated by one- and two-dimensional gel electrophoresis and Western blot analysis. Immunohistochemistry was performed on 25 human eyes enucleated because of posterior choroidal melanoma and on 7 normal human donor eyes.
RESULTS. By Western blot analysis, a band at approximately 57 kDa was visualized in cornea, trabecular meshwork, lamina cribrosa, optic nerve, retina, iris, ciliary body, and vitreous humor. By immunohistochemistry, immunoreactivity for myocilin/TIGR was observed in cells of the corneal epi- and endothelium and extracellularly in the corneal stroma and sclera. In the trabecular meshwork, cells of the uveal and corneoscleral meshwork were stained, as was the cribriform area directly adjacent to Schlemm’s canal. Positive staining was seen in cells of the ciliary epithelium, ciliary muscle, lens epithelium, and in stromal and smooth muscle cells of the iris. Throughout the entire vitreous body, fine filamentous material was positively labeled. In the retina, staining was seen along the outer surface of rods and cones, in neurons of the inner and outer nuclear layer, and in the axons of optic nerve ganglion cells. Optic nerve axons were stained in the prelaminar, laminar, and postlaminar parts of the nerve. In the region of the lamina cribrosa, astrocytes in the glial columns and cribriform plates were positively labeled.
CONCLUSIONS. Myocilin/TIGR is expressed in almost every ocular tissue. Depending on the respective tissue, it is observed extra- or intracellularly. The presence of myocilin/TIGR in optic nerve axons and lamina cribrosa astrocytes indicates that the trabecular meshwork might not be the only target of abnormal myocilin/TIGR in GLC1A-linked open-angle glaucoma.
This article has been cited by other articles:
|
|
 |
|
  P. Russell, J. Z. Gasiorowski, P. F. Nealy, and C. J. Murphy Response of Human Trabecular Meshwork Cells to Topographic Cues on the Nanoscale Level Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 629 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Sanchez-Sanchez, F. Martinez-Redondo, J. D. Aroca-Aguilar, M. Coca-Prados, and J. Escribano Characterization of the Intracellular Proteolytic Cleavage of Myocilin and Identification of Calpain II as a Myocilin-processing Protease J. Biol. Chem., September 21, 2007; 282(38): 27810 - 27824. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. H.-F. Yam, K. Gaplovska-Kysela, C. Zuber, and J. Roth Sodium 4-Phenylbutyrate Acts as a Chemical Chaperone on Misfolded Myocilin to Rescue Cells from Endoplasmic Reticulum Stress and Apoptosis Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1683 - 1690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. H.-F. Yam, K. Gaplovska-Kysela, C. Zuber, and J. Roth Aggregated Myocilin Induces Russell Bodies and Causes Apoptosis: Implications for the Pathogenesis of Myocilin-Caused Primary Open-Angle Glaucoma Am. J. Pathol., January 1, 2007; 170(1): 100 - 109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Gould, M. Reedy, L. A. Wilson, R. S. Smith, R. L. Johnson, and S. W. M. John Mutant Myocilin Nonsecretion In Vivo Is Not Sufficient To Cause Glaucoma Mol. Cell. Biol., November 15, 2006; 26(22): 8427 - 8436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Malyukova, H.-S. Lee, R. N. Fariss, and S. I. Tomarev Mutated Mouse and Human Myocilins Have Similar Properties and Do Not Block General Secretory Pathway Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 206 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Fautsch, C. K. Bahler, A. M. Vrabel, K. G. Howell, N. Loewen, W. L. Teo, E. M. Poeschla, and D. H. Johnson Perfusion of His-Tagged Eukaryotic Myocilin Increases Outflow Resistance in Human Anterior Segments in the Presence of Aqueous Humor Invest. Ophthalmol. Vis. Sci., January 1, 2006; 47(1): 213 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Aroca-Aguilar, F. Sanchez-Sanchez, S. Ghosh, M. Coca-Prados, and J. Escribano Myocilin Mutations Causing Glaucoma Inhibit the Intracellular Endoproteolytic Cleavage of Myocilin between Amino Acids Arg226 and Ile227 J. Biol. Chem., June 3, 2005; 280(22): 21043 - 21051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Aung, V. H. K. Yong, P. T. K. Chew, S. K. L. Seah, G. Gazzard, P. J. Foster, and E. N. Vithana Molecular Analysis of the Myocilin Gene in Chinese Subjects with Chronic Primary-Angle Closure Glaucoma Invest. Ophthalmol. Vis. Sci., April 1, 2005; 46(4): 1303 - 1306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zillig, A. Wurm, F. J. Grehn, P. Russell, and E. R. Tamm Overexpression and Properties of Wild-Type and Tyr437His Mutated Myocilin in the Eyes of Transgenic Mice Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 223 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Zhao, K. E. Ramsey, D. A. Stephan, and P. Russell Gene and Protein Expression Changes in Human Trabecular Meshwork Cells Treated with Transforming Growth Factor-{beta} Invest. Ophthalmol. Vis. Sci., November 1, 2004; 45(11): 4023 - 4034. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Gould, L. Miceli-Libby, O. V. Savinova, M. Torrado, S. I. Tomarev, R. S. Smith, and S. W. M. John Genetically Increasing Myoc Expression Supports a Necessary Pathologic Role of Abnormal Proteins in Glaucoma Mol. Cell. Biol., October 15, 2004; 24(20): 9019 - 9025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gobeil, M.-A. Rodrigue, S. Moisan, T. D. Nguyen, J. R. Polansky, J. Morissette, and V. Raymond Intracellular Sequestration of Hetero-oligomers Formed by Wild-Type and Glaucoma-Causing Myocilin Mutants Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3560 - 3567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Goldwich, C. R. Ethier, D. W.-H. Chan, and E. R. Tamm Perfusion with the Olfactomedin Domain of Myocilin Does Not Affect Outflow Facility Invest. Ophthalmol. Vis. Sci., May 1, 2003; 44(5): 1953 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sohn, W. Hur, M. K. Joe, J.-H. Kim, Z.-W. Lee, K.-S. Ha, and C. Kee Expression of Wild-Type and Truncated Myocilins in Trabecular Meshwork Cells: Their Subcellular Localizations and Cytotoxicities Invest. Ophthalmol. Vis. Sci., December 1, 2002; 43(12): 3680 - 3685. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Filla, X. Liu, T. D. Nguyen, J. R. Polansky, C. R. Brandt, P. L. Kaufman, and D. M. Peters In Vitro Localization of TIGR/MYOC in Trabecular Meshwork Extracellular Matrix and Binding to Fibronectin Invest. Ophthalmol. Vis. Sci., January 1, 2002; 43(1): 151 - 161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wentz-Hunter, J. Ueda, and B. Y. J. T. Yue Protein Interactions with Myocilin Invest. Ophthalmol. Vis. Sci., January 1, 2002; 43(1): 176 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Ahmed, M. Torrado, E. Johnson, J. Morrison, and S. I. Tomarev Changes in mRNA Levels of the Myoc/Tigr Gene in the Rat Eye after Experimental Elevation of Intraocular Pressure or Optic Nerve Transection Invest. Ophthalmol. Vis. Sci., December 1, 2001; 42(13): 3165 - 3172. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Kim, O. V. Savinova, M. V. Reedy, J. Martin, Y. Lun, L. Gan, R. S. Smith, S. I. Tomarev, S. W. M. John, and R. L. Johnson Targeted Disruption of the Myocilin Gene (Myoc) Suggests that Human Glaucoma-Causing Mutations Are Gain of Function Mol. Cell. Biol., November 15, 2001; 21(22): 7707 - 7713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Fautsch and D. H. Johnson Characterization of Myocilin-Myocilin Interactions Invest. Ophthalmol. Vis. Sci., September 1, 2001; 42(10): 2324 - 2331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Lindsey, D. D. Gaton, T. Sagara, J. R. Polansky, P. L. Kaufman, and R. N. Weinreb Reduced TIGR/Myocilin Protein in the Monkey Ciliary Muscle after Topical Prostaglandin F2{{alpha}} Treatment Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1781 - 1786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Russell, E. R. Tamm, F. J. Grehn, G. Picht, and M. Johnson The Presence and Properties of Myocilin in the Aqueous Humor Invest. Ophthalmol. Vis. Sci., April 1, 2001; 42(5): 983 - 986. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Jacobson, M. Andrews, A. R. Shepard, D. Nishimura, C. Searby, J. H. Fingert, G. Hageman, R. Mullins, B. L. Davidson, Y. H. Kwon, et al. Non-secretion of mutant proteins of the glaucoma gene myocilin in cultured trabecular meshwork cells and in aqueous humor Hum. Mol. Genet., January 1, 2001; 10(2): 117 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. P. Fautsch, C. K. Bahler, D. J. Jewison, and D. H. Johnson Recombinant TIGR/MYOC Increases Outflow Resistance in the Human Anterior Segment Invest. Ophthalmol. Vis. Sci., December 1, 2000; 41(13): 4163 - 4168. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. Ueda, K. K. Wentz–Hunter, E. L. Cheng, T. Fukuchi, H. Abe, and B. Y.J.T. Yue Ultrastructural Localization of Myocilin in Human Trabecular Meshwork Cells and Tissues J. Histochem. Cytochem., October 1, 2000; 48(10): 1321 - 1330. [Abstract] [Full Text]
|
|
|
|
|
|
R. E. Swiderski, J. L. Ross, J. H. Fingert, A. F. Clark, W. L. M. Alward, E. M. Stone, and V. C. Sheffield Localization of MYOC Transcripts in Human Eye and Optic Nerve by In Situ Hybridization Invest. Ophthalmol. Vis. Sci., October 1, 2000; 41(11): 3420 - 3428. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
