IOVS Molecular Human Reproduction
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Quinn, R. H.
Right arrow Articles by Miller, S. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quinn, R. H.
Right arrow Articles by Miller, S. S.
(Investigative Ophthalmology and Visual Science. 2001;42:255-264.)
© 2001 by The Association for Research in Vision and Ophthalmology, Inc.

Adrenergic Receptor Activated Ion Transport in Human Fetal Retinal Pigment Epithelium

Richard H. Quinn1,2, Judy N. Quong1,3 and Sheldon S. Miller1,4

1 From the School of Optometry 2 Department of Molecular and Cell Biology, University of California, Berkeley 3 Department of Zoology, University of Texas, Austin 4 Department of Medicine, Division of Hematology/Oncology, University of California, San Francisco.

PURPOSE. To identify the apical and basolateral membrane mechanisms and intracellular signaling pathways in human fetal retinal pigment epithelium (HRPE) that mediate membrane voltage and resistance changes caused by apical membrane adrenergic receptor activation.

METHODS. Intact sheets of RPE-choroid from human fetal eyes were mounted in a modified Ussing chamber. Ringer’s solution composition changes on the retina-facing and choroid-facing sides of the tissue were separately controlled. Intracellular microelectrodes recorded the membrane voltage and resistance changes after the addition of pharmacologic agents to the apical or basal baths.

RESULTS. Apical adrenergic agonists, isoproterenol and epinephrine (10-8 M), depolarized the basolateral membrane, decreased total tissue resistance (Rt) and increased the ratio of apical-to-basolateral membrane resistance (RA/RB). Experiments using antagonists for {alpha}1 and ß adrenergic receptors, prazosin and propranolol, respectively, indicated that both receptor types were present. The epinephrine responses were inhibited by apical bumetanide and basal 4,4'-diisothiocyanostilbene-2,2' disulfonic acid (DIDS). A cocktail of cyclic adenosine monophosphate (cAMP)–elevating agents produced basolateral membrane voltage and resistance changes very similar to the isoproterenol responses. The cAMP-induced electrical responses were strongly inhibited by basal 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB). Ionomycin (to elevate intercellular Ca2+, [Ca2+]i) produced electrical responses similar to those caused by epinephrine. The Ca2+ responses were unaffected by NPPB but were inhibited by 3 mM DIDS in the basal bath.

CONCLUSIONS. The results provide evidence for two apical membrane adrenergic receptors, {alpha}1 and ß, activated by epinephrine and isoproterenol, respectively. The membrane voltage and resistance changes produced by these two agonists mimic those produced by elevating [Ca2+]i and [cAMP]i, suggesting that these ubiquitous signaling molecules activate separate basolateral membrane Cl channels inhibited by DIDS and NPPB, respectively. These two receptors, the apical membrane NaK2Cl cotransporters and the basolateral membrane Cl channels form a complex of proteins that help mediate fluid absorption across human RPE.




This article has been cited by other articles:


Home page
 J. Neurophysiol.Home page
J. Wu, A. D. Marmorstein, J. Striessnig, and N. S. Peachey
Voltage-Dependent Calcium Channel CaV1.3 Subunits Regulate the Light Peak of the Electroretinogram
J Neurophysiol, May 1, 2007; 97(5): 3731 - 3735.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
D. Reigada, W. Lu, and C. H. Mitchell
Glutamate acts at NMDA receptors on fresh bovine and on cultured human retinal pigment epithelial cells to trigger release of ATP
J. Physiol., September 15, 2006; 575(3): 707 - 720.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Maminishkis, S. Chen, S. Jalickee, T. Banzon, G. Shi, F. E. Wang, T. Ehalt, J. A. Hammer, and S. S. Miller
Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue.
Invest. Ophthalmol. Vis. Sci., August 1, 2006; 47(8): 3612 - 3624.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
L. A. Voloboueva, J. Liu, J. H. Suh, B. N. Ames, and S. S. Miller
(R)-{alpha}-Lipoic Acid Protects Retinal Pigment Epithelial Cells from Oxidative Damage
Invest. Ophthalmol. Vis. Sci., November 1, 2005; 46(11): 4302 - 4310.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
C. Hartzell, Z. Qu, I. Putzier, L. Artinian, L.-T. Chien, and Y. Cui
Looking Chloride Channels Straight in the Eye: Bestrophins, Lipofuscinosis, and Retinal Degeneration
Physiology, October 1, 2005; 20(5): 292 - 302.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
O. Strauss
The Retinal Pigment Epithelium in Visual Function
Physiol Rev, July 1, 2005; 85(3): 845 - 881.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
D. Reigada and C. H. Mitchell
Release of ATP from retinal pigment epithelial cells involves both CFTR and vesicular transport
Am J Physiol Cell Physiol, January 1, 2005; 288(1): C132 - C140.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. Wu, N. S. Peachey, and A. D. Marmorstein
Light-Evoked Responses of the Mouse Retinal Pigment Epithelium
J Neurophysiol, March 1, 2004; 91(3): 1134 - 1142.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. E. Loewen, N. K. Smith, D. L. Hamilton, B. H. Grahn, and G. W. Forsyth
CLCA protein and chloride transport in canine retinal pigment epithelium
Am J Physiol Cell Physiol, November 1, 2003; 285(5): C1314 - C1321.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
H. J. Gukasyan, K.-J. Kim, R. Kannan, R. A. Farley, and V. H. L. Lee
Specialized Protective Role of Mucosal Glutathione in Pigmented Rabbit Conjunctiva
Invest. Ophthalmol. Vis. Sci., October 1, 2003; 44(10): 4427 - 4438.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
S. Blaug, J. Rymer, S. Jalickee, and S. S. Miller
P2 purinoceptors regulate calcium-activated chloride and fluid transport in 31EG4 mammary epithelia
Am J Physiol Cell Physiol, April 1, 2003; 284(4): C897 - C909.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. Maminishkis, S. Jalickee, S. A. Blaug, J. Rymer, B. R. Yerxa, W. M. Peterson, and S. S. Miller
The P2Y2 Receptor Agonist INS37217 Stimulates RPE Fluid Transport In Vitro and Retinal Reattachment in Rat
Invest. Ophthalmol. Vis. Sci., November 1, 2002; 43(11): 3555 - 3566.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
T. X. Weng, B. F. Godley, G. F. Jin, N. J. Mangini, B. G. Kennedy, A. S. L. Yu, and N. K. Wills
Oxidant and antioxidant modulation of chloride channels expressed in human retinal pigment epithelium
Am J Physiol Cell Physiol, September 1, 2002; 283(3): C839 - C849.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. Rymer, S. S. Miller, and J. L. Edelman
Epinephrine-Induced Increases in [Ca2+]in and KCl-Coupled Fluid Absorption in Bovine RPE
Invest. Ophthalmol. Vis. Sci., July 1, 2001; 42(8): 1921 - 1929.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the Association for Research in Vision and Ophthalmology