| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 From the Universitätsklinikum Eppendorf (UKE) Augenklinik and 2 Zentrum für Molekulare Neurobiologie, Universität Hamburg, Germany.
PURPOSE. To study the integration and differentiation of heterotopically transplanted neural precursor cells in the retina of adult mouse mutants displaying apoptotic degeneration of photoreceptor cells.
METHODS. Neural precursor cells were isolated from the spinal cord of transgenic mouse embryos ubiquitously expressing enhanced green fluorescent protein. Cells were expanded in vitro and transplanted into the retina of adult wild-type and age-matched ß2/ß1 knock-in mice. ß2/ß1 knock-in mutants display apoptotic death of photoreceptor cells and were generated by placing the cDNA of the ß1 subunit into the gene of the ß2 subunit of Na,K-ATPase. The integration and differentiation of grafted cells in recipient retinas was studied 1 or 6 months after transplantation.
RESULTS. Mutant retinas contained more donor-derived cells than wild-type hosts. Moreover, in mutants, donor cells integrated into deeper retinal layers. In both genotypes, grafted cells differentiated into astrocytes and oligodendrocytes. Only a few ganglion cell axons were myelinated by donor-derived oligodendrocytes 1 month after transplantation, whereas extensive myelination of the nerve fiber layer was observed 6 months after transplantation. Unequivocal evidence for differentiation of grafted cells into neurons was not obtained.
CONCLUSIONS. Heterotopically transplanted neural precursor cells are capable of integrating, surviving, and differentiating into neural cell types in normal and dystrophic retinas of adult mice. The particular environment of a pathologically altered retina facilitates integration of transplanted precursor cells. In principle, neural precursors may thus be useful to substitute for or replace dysfunctional or degenerated cell types. Results of the present study also indicate that replacement of retinal cell types is likely to require more appropriate donor cells, such as retinal precursor cells.
This article has been cited by other articles:
|
|
 |
|
  K. Canola, B. Angenieux, M. Tekaya, A. Quiambao, M. I. Naash, F. L. Munier, D. F. Schorderet, and Y. Arsenijevic Retinal Stem Cells Transplanted into Models of Late Stages of Retinitis Pigmentosa Preferentially Adopt a Glial or a Retinal Ganglion Cell Fate Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 446 - 454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Banin, A. Obolensky, M. Idelson, I. Hemo, E. Reinhardtz, E. Pikarsky, T. Ben-Hur, and B. Reubinoff Retinal Incorporation and Differentiation of Neural Precursors Derived from Human Embryonic Stem Cells Stem Cells, February 1, 2006; 24(2): 246 - 257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Meyer, M. L. Katz, J. A. Maruniak, and M. D. Kirk Embryonic Stem Cell-Derived Neural Progenitors Incorporate into Degenerating Retina and Enhance Survival of Host Photoreceptors Stem Cells, February 1, 2006; 24(2): 274 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Arnhold, H. Klein, I. Semkova, K. Addicks, and U. Schraermeyer Neurally Selected Embryonic Stem Cells Induce Tumor Formation after Long-Term Survival following Engraftment into the Subretinal Space Invest. Ophthalmol. Vis. Sci., December 1, 2004; 45(12): 4251 - 4255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. B. Wojciechowski, U. Englund, C. Lundberg, and K. Warfvinge Survival and Long Distance Migration of Brain-Derived Precursor Cells Transplanted to Adult Rat Retina Stem Cells, January 1, 2004; 22(1): 27 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kicic, W.-Y. Shen, A. S. Wilson, I. J. Constable, T. Robertson, and P. E. Rakoczy Differentiation of Marrow Stromal Cells into Photoreceptors in the Rat Eye J. Neurosci., August 27, 2003; 23(21): 7742 - 7749. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
