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(Investigative Ophthalmology and Visual Science. 2002;43:3196-3201.)
© 2002 by The Association for Research in Vision and Ophthalmology, Inc.

Progress in the Development of a Synthetic Corneal Onlay

Margaret D. M. Evans1,2, Ruo Zhong Xie1, Mirella Fabbri1, Barbara Bojarski1, Hassan Chaouk1,3, John S. Wilkie1,3, Keith M. McLean1,3, Han Ying Cheng1,4, Antti Vannas1,5 and Deborah F. Sweeney1

1 From the Cooperative Research Centre for Eye Research and Technology and the 4 Graduate School for Biomedical Engineering, University of New South Wales, Sydney, Australia; 2 CSIRO Molecular Science, North Ryde, Sydney, Australia; 3 CSIRO Molecular Science, Clayton, Melbourne, Australia; and the 5 Department of Ophthalmology, Central Military Hospital, Helsinki, Finland.

PURPOSE. This study evaluated an improved perfluoropolyether polymer formulation designed for use as a corneal onlay to correct refractive error.

METHODS. Collagen I coated perfluoropolyether lenticules were implanted in feline corneas exposing a 6-mm diameter area of lenticule surface for epithelial growth. A parallel series of sham-wounded corneas were also studied. All corneas were monitored clinically for 4 or 8 weeks after surgery when animals were terminated and corneas used for histology with light and electron microscopy.

RESULTS. Postoperative epithelial growth began on days 1 and 2. Lenticule surfaces were fully epithelialized by days 5 to 11. Corneas remained clear, and the lenticules maintained epithelial cover until the designated time points. Histology of the implanted corneas showed that the lenticules were well tolerated by the cornea. Each lenticule was fully covered by a multilayered epithelium with microvilli, desmosomes, and a differentiated basal cell layer. Epithelial adhesive structures (basal lamina, hemidesmosomes, and anchoring fibrils) had assembled at the tissue–lenticule interface.

CONCLUSIONS. Collagen coated perfluoropolyether lenticules implanted in the feline cornea supported the growth of a stable stratified squamous epithelium. These encouraging results are a step further in the development of a corneal onlay for correction of refractive error.




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R. Z. Xie, M. D. M. Evans, B. Bojarski, T. C. Hughes, G. Y. Chan, X. Nguyen, J. S. Wilkie, K. M. McLean, A. Vannas, and D. F. Sweeney
Two-Year Preclinical Testing of Perfluoropolyether Polymer as a Corneal Inlay
Invest. Ophthalmol. Vis. Sci., February 1, 2006; 47(2): 574 - 581.
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