IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

(Investigative Ophthalmology and Visual Science. 2003;44:5168-5173.)
© 2003 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.03-0504
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aihara, M.
Right arrow Articles by Weinreb, R. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aihara, M.
Right arrow Articles by Weinreb, R. N.

Aqueous Humor Dynamics in Mice

Makoto Aihara, James D. Lindsey, and Robert N. Weinreb

From the Hamilton Glaucoma Center and Department of Ophthalmology, University of California San Diego, La Jolla, California.

PURPOSE. To assess aqueous humor dynamics in mouse eyes.

METHODS. Aqueous humor dynamics of NIH Swiss White mouse were assessed with an injection and aspiration system, using fine glass microneedles. Intraocular pressure (IOP) was measured by a microneedle connected to a pressure transducer. Episcleral venous pressure (EVP) was measured by gradually lowering intracameral pressure until blood reflux into Schlemm’s canal was observed. Outflow facility (C) was determined based on constant pressure perfusion measurements obtained at two different IOPs. Aqueous volume (Va) was determined by direct measurement of aspirated aqueous humor. Aqueous humor production (Fa) was measured by the dilution method with rhodamine-dextran. Conventional and uveoscleral outflow (Fc and Fu, respectively), as well as the turnover rate of aqueous humor, were also calculated.

RESULTS. IOP and EVP were 15.7 ± 2.0 and 9.5 ± 1.2 mm Hg, respectively (n = 20). Fa was 0.18 ± 0.05 µL/min (mean ± SD; n = 8). C was 0.0051 ± 0.0006 µL/min per mm Hg (n = 8). Estimated Fc and Fu were 0.032 and 0.148 µL/min, respectively. Fc was 18% of Fa. Fu was 82% of Fa. Va was 5.9 ± 0.5 µL (n = 8). The calculated turnover rate of aqueous humor was 2.5%.

CONCLUSIONS. The mouse eye has similar aqueous production and aqueous humor turnover rate as the human eye. The presence of both conventional and uveoscleral outflow suggests that the mouse is a useful model system for further investigations of the biology of aqueous dynamics.




This article has been cited by other articles:


Home page
 IOVSHome page
B. Bakall, P. McLaughlin, J. B. Stanton, Y. Zhang, H. C. Hartzell, L. Y. Marmorstein, and A. D. Marmorstein
Bestrophin-2 Is Involved in the Generation of Intraocular Pressure
Invest. Ophthalmol. Vis. Sci., April 1, 2008; 49(4): 1563 - 1570.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. G. Crowston, C. A. Morris, J. D. Lindsey, and R. N. Weinreb
Prostaglandin FP Receptors Do Not Contribute to 24-hour Intraocular Pressure Variation in Mice
Invest. Ophthalmol. Vis. Sci., May 1, 2007; 48(5): 2095 - 2098.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
L. Zhong, J. Bradley, W. Schubert, E. Ahmed, A. P. Adamis, D. T. Shima, G. S. Robinson, and Y.-S. Ng
Erythropoietin Promotes Survival of Retinal Ganglion Cells in DBA/2J Glaucoma Mice
Invest. Ophthalmol. Vis. Sci., March 1, 2007; 48(3): 1212 - 1218.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. P. Fautsch, D. H. Johnson, and the Second ARVO/Pfizer Research Institute Working
Aqueous humor outflow: what do we know? Where will it lead us?
Invest. Ophthalmol. Vis. Sci., October 1, 2006; 47(10): 4181 - 4187.
[Full Text] [PDF]

Home page
 IOVSHome page
J. G. Crowston, J. D. Lindsey, C. A. Morris, L. Wheeler, F. A. Medeiros, and R. N. Weinreb
Effect of Bimatoprost on Intraocular Pressure in Prostaglandin FP Receptor Knockout Mice
Invest. Ophthalmol. Vis. Sci., December 1, 2005; 46(12): 4571 - 4577.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
T. Ota, H. Murata, E.-i. Sugimoto, M. Aihara, and M. Araie
Prostaglandin Analogues and Mouse Intraocular Pressure: Effects of Tafluprost, Latanoprost, Travoprost, and Unoprostone, Considering 24-Hour Variation
Invest. Ophthalmol. Vis. Sci., June 1, 2005; 46(6): 2006 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. Zillig, A. Wurm, F. J. Grehn, P. Russell, and E. R. Tamm
Overexpression and Properties of Wild-Type and Tyr437His Mutated Myocilin in the Eyes of Transgenic Mice
Invest. Ophthalmol. Vis. Sci., January 1, 2005; 46(1): 223 - 234.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. G. Crowston, J. D. Lindsey, M. Aihara, and R. N. Weinreb
Effect of Latanoprost on Intraocular Pressure in Mice Lacking the Prostaglandin FP Receptor
Invest. Ophthalmol. Vis. Sci., October 1, 2004; 45(10): 3555 - 3559.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. G. Crowston, M. Aihara, J. D. Lindsey, and R. N. Weinreb
Effect of Latanoprost on Outflow Facility in the Mouse
Invest. Ophthalmol. Vis. Sci., July 1, 2004; 45(7): 2240 - 2245.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
F. Mabuchi, J. D. Lindsey, M. Aihara, M. R. Mackey, and R. N. Weinreb
Optic Nerve Damage in Mice with a Targeted Type I Collagen Mutation
Invest. Ophthalmol. Vis. Sci., June 1, 2004; 45(6): 1841 - 1845.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2003 by the Association for Research in Vision and Ophthalmology