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(Investigative Ophthalmology and Visual Science. 2004;45:3849-3856.)
© 2004 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.04-0496
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Interaction of Lumican with Aggrecan in the Aging Human Sclera

Jane R. Dunlevy1 and Jody A. Summers Rada2

1From the Department of Anatomy and Cell Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, North Dakota; and the 2Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma.

PURPOSE. Lumican is a keratan sulfate proteoglycan originally identified in cornea, but present in a variety of connective tissues where it presumably regulates collagen fibril formation and organization. The present study was designed to describe the chemical nature of lumican core protein in the aging human sclera.

METHODS. Western blot analyses, immunohistochemistry, and immunoaffinity chromatography were used to detect and purify the lumican core protein from tissue extracts from human donors 6 to 89 years of age. Treatment of lumican with chondroitinase ABC, keratanase-I and -II, and/or endo-ß-galactosidase was used to determine the degree of glycosylation of the lumican core protein.

RESULTS. Lumican was present in the human sclera as a 70- to 80-kDa core protein with short unsulfated lactosaminoglycan side chains. In addition, on Western blots, a larger >200-kDa species was apparent that was immunologically related to lumican. This high-molecular-weight material increased in scleral extracts with increasing age. The complex was most abundant in unreduced samples, and approximately two thirds of the 70- to 80-kDa lumican core protein was released from the complex on reduction of the scleral extract. Further characterization of the >200-kDa lumican-immunopurified complex indicated that aggrecan (the cartilage proteoglycan) was covalently associated with lumican.

CONCLUSIONS. Reducible and nonreducible lumican-aggrecan interactions occur in the scleral extracellular matrix and result in the formation of high-molecular-weight complexes that increase with age. These results represent the first report demonstrating lumican-aggrecan interactions and suggest they may play a role in age-related scleral extracellular matrix changes.




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Y. Kaji, T. Oshika, Y. Takazawa, M. Fukayama, T. Takata, and N. Fujii
Localization of D-{beta}-Aspartic Acid Containing Proteins in Human Eyes
Invest. Ophthalmol. Vis. Sci., September 1, 2007; 48(9): 3923 - 3927.
[Abstract] [Full Text] [PDF]


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