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Cell Cycle–Specific and Cell Type–Specific Expression of Rb in the Developing Human Retina

¹From the Margaret M. Dyson Vision Research Institute, and ²Department of Ophthalmology, Weill Medical College of Cornell University, New York, New York; and the ⁴Ophthalmic Oncology Service, Department of Surgery, Memorial Sloan-Kettering Cancer Center, New York, New York.

PURPOSE. To define the pattern of Rb expression relative to cell cycle position and cell type in the developing human retina.

METHODS. Cryosections of fetal week 11-18 retinas were immunostained for Rb and cell cycle– or cell type–specific markers.

RESULTS. Rb was prominent in retinal progenitor cells (RPCs) expressing the cyclin D1, cyclin A, and cytoplasmic cyclin B markers of G₁, S, and early to mid G₂ phases, but not in RPCs expressing the phosphohistone H3 marker of late G₂ and M. Rb was not detected in the earliest postmitotic ganglion, amacrine, horizontal, and bipolar cell precursors migrating away from the ventricular layer, but was detected as such cells underwent further differentiation. Among photoreceptors, Rb was not detected in the earliest RXR(+) cone precursors or in the earliest Nrl(+) rod precursors, but subsequently rose to high levels in cones and to low levels in rods. Rb was prominent at the time when Müller glia exit the cell cycle and was generally expressed in a pattern complementary to p27^Kip1.

CONCLUSIONS. Rb exhibits cell cycle–specific expression in RPCs, with loss in late G₂-M and restoration in G₁. Rb is re-expressed after postmitotic ganglion, amacrine, horizontal, and bipolar cell precursors migrate away from the ventricular layer; after the appearance of early cone and rod markers; but coinciding with Müller glia cell cycle withdrawal. The results suggest that Rb does not mediate the initial proliferative arrest of retinal neurons, but may indirectly induce arrest in RPCs or maintain an arrest in postmitotic precursors.

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