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(Investigative Ophthalmology and Visual Science. 2006;47:3745-3753.)
© 2006 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.06-0086
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Light-Driven Cone Arrestin Translocation in Cones of Postnatal Guanylate Cyclase-1 Knockout Mouse Retina Treated with AAV-GC1

Shannon E. Haire,1 Jijing Pang,2 Sanford L. Boye,2 Izabel Sokal,3 Cheryl M. Craft,4 Krzysztof Palczewski,5 William W. Hauswirth,2 and Susan L. Semple-Rowland1

1From the Department of Neuroscience, McKnight Brain Institute, and the 2Department of Ophthalmology, University of Florida, Gainesville, Florida; the 3Department of Ophthalmology and Pharmacology, University of Washington, Seattle, Washington; the 4Mary D. Allen Laboratory for Vision Research, Doheny Eye Institute, Department of Ophthalmology and Cell and Neurobiology, Keck Medical School of Medicine of the University of Southern California, Los Angeles, California; and the 5Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio.

PURPOSE. Cone function and survival are compromised in the guanylate cyclase-1 (GC1) knockout mouse. Disruption of the light-driven translocation of cone arrestin is one of the phenotypes of cone cells in this retina: the cone arrestin in these cells is localized to the outer segments and synaptic terminals, regardless of the state of light adaptation. The purpose of this study was to determine whether the expression of GC1 restores cone arrestin translocation in the cone cells of postnatal GC1 knockout mouse retina.

METHODS. Subretinal injections of AAV-GC1 were performed on 3-week-old GC1 KO mice. Electroretinographic and immunohistochemical analyses of treated retinas were carried out 5 weeks after injection. GC1 and cone arrestin antibodies were used to identify photoreceptors transduced by the AAV vector and to localize cone arrestin within cone cells, respectively.

RESULTS. Treatment of GC1 knockout retinas with AAV-GC1 restored the light-driven translocation of cone arrestin in transduced cone cells. Staining patterns for cone arrestin in transduced and wild-type cone cells were indistinguishable after dark and light adaptation. In dark-adapted retinas, cone arrestin was distributed throughout the subcellular compartments of the cone cells. In light-adapted retinas, cone arrestin was concentrated in the cone outer segments. Successful restoration of cone arrestin translocation did not translate to a restoration of cone ERG responses, which remained undetectable in the treated retinas.

CONCLUSIONS. AAV-mediated expression of GC1 in a subpopulation of cone cells in postnatal GC1 knockout retina restores light-driven translocation of cone arrestin in these cells. These findings, which show that fully developed cone cells that have developed in the absence of GC1 can respond to viral-mediated expression of this enzyme, support further analysis of this animal model of Leber congenital amaurosis type 1 (LCA1), a disease that results from null mutations in the gene encoding this enzyme.




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