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(Investigative Ophthalmology and Visual Science. 1966;5:14-21.)
© 1966 by The Association for Research in Vision and Ophthalmology, Inc.

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The Development of Enzyme Activities in Corneal Connective Tissue Cells During the Lag Phase of Wound Repair: II. Formalin-Resistant Oxidase-Like Reaction

VIRGINIA L. WEIMAR 1 and KENNETH H. HARAGUCHI 1

1 John E. Weeks Memorial Laboratory, Department of Ophthalmology, University of Oregon Medical School, Portland, Ore.

A formalin-resistant oxidase-like reaction which is cyanide- and phenylhydrazine-sensitive, but azide insensitive, develops intense activity in corneal endothelial, epithelial, and connective tissue cells within 24 hours after injury to the rat cornea. This apparently new oxidase-like system cannot be detected in the normal cornea. It attains maximal activity between 24 and 48 hours after injury and disappears between the fourth and seventh postoperative days. No cytochrome oxidase activity could be demonstrated in either the normal or in the healing cornea.






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