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(Investigative Ophthalmology and Visual Science. 1968;7:357-365.)
© 1968 by The Association for Research in Vision and Ophthalmology, Inc.

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In Vitro Neoplastic Transformation of Uveal and Retinal Tissue by Oncogenic DNA Viruses

DANIEL M. ALBERT 1, ALAN S. RABSON 2, and ALBERT J. DALTON 2

1 Ophthalmology Branch, National Institute of Neurological Diseases and Blindness; Pathological Anatomy Branch and Viral Biology Branch, National Cancer Institute, National Institutes of Health, Public Health Service, United States Department of Health, Education and Welfare Bethesda, Md. 20014.; Ophthalmic Pathology Branch, Armed Forces Institute of Pathology, Washington, D.C.
2 Ophthalmology Branch, National Institute of Neurological Diseases and Blindness; Pathological Anatomy Branch and Viral Biology Branch, National Cancer Institute, National Institutes of Health, Public Health Service, United States Department of Health, Education and Welfare Bethesda, Md. 20014.

Explant cultures of adult hamster retina, choroid, and iris were infected with four oncogenic DNA viruses: (1) simian virus 40 (SV40); (2) the LLE46 strain of adenovirus 7; (3) polyoma virus; and (4) human adenovirus 12. Most cidtures underwent transformation as evidenced by sustained rapid growth, development of an acid pH soon after instillation of fresh media, and. formation of multiple layers of cells and clumps. After several in vitro passages, transformed cells injected subcutaneously into irradiated adult hamsters produced tumors at the injection sites. All ocular tissue transformed with SV40 and produced neoplasms composed of epithelioid and spindle-shaped cells with large nuclei; however, no pigmented tumors were observed. Choroid and iris transformed with LLE46 produced tumors histologically similar to the SV40-type growths. Retina infected with LLE46 produced tumors which were bimorphic, containing, in addition to the SV40-like component, areas of small hyperchromatic cells resembling an adenovirus-induced tumor. Polyoma virus transformed only retina, producing tumors with elongated cells tuith hyperchromatic pleomorphic nuclei and small amounts of basophilic cytoplasm. Adenovirus 12 also transformed only retina and gave rise to typical adenovirus-type neoplasms composed of layers of small, darkly staining cells. Although these tumors had some resemblance to retinoblastomas, no rosettes or neural elements could be identified.




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N Mukai, S. Kalter, L. Cummins, V. Matthews, T Nishida, and T Nakajima
Retinal tumor induced in the baboon by human adenovirus 12
Science, November 28, 1980; 210(4473): 1023 - 1025.
[Abstract] [PDF]

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D. M. Albert, A. S. Rabson, P. A. Grimes, and L. von Sallmann
Neoplastic Transformation in vitro of Hamster Lens Epithelium by Simian Virus 40
Science, May 30, 1969; 164(3883): 1077 - 1078.
[Abstract] [PDF]


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