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(Investigative Ophthalmology and Visual Science. 1968;7:435-440.)
© 1968 by The Association for Research in Vision and Ophthalmology, Inc.

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Rabbit Tear Proteins. I. Detection and Quantitation of Lysozyme in Nonstimulated Tears

BENJAMIN BONAVIDA 1, ALFRED T. SAPSE 1, and ELI E. SERCARZ 1

1 Department of Bacteriology, University of California Los Angeles, Calif. 90024.

Rabbit tear lysozyme was detected in low concentration in rabbit tears collected without any stimulation. Quantitation of rabbit tear lysozyme was found possible through the use of sensitive techniques that measure its specific enzymic activity on the bacterial cell walls of Micrococcus lysodeikticus. Rabbit tear lysozyme has an electrophoretic mobility identical to that of hen egg-white lysozyme on cellulose and acrylamide gel. The experimental use of rabbits for tear lysozyme studies is discussed.

Note:

Recipient of United States Public Health Traineeship No. 5T01-GM-01297-02.

Recipient of Public Health Service Special Fellowship No. 1F11 NB1651-01.






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Copyright © 1968 by the Association for Research in Vision and Ophthalmology