IOVS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

P<P, published online ahead of print April 25, 2008
(Investigative Ophthalmology and Visual Science. )
© 2008 by The Association for Research in Vision and Ophthalmology, Inc.
DOI:  10.1167/iovs.08-1691
This Article
Right arrow Full Text (P<P[PDF])
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Ueta, M.
Right arrow Articles by Kinoshita, S.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ueta, M.
Right arrow Articles by Kinoshita, S.

Article

Stat6-independent tissue inflammation occurs selectively on the ocular surface and perioral skin of I{kappa}B{zeta}-/- mice

Mayumi Ueta 1*, Junji Hamuro 1, Eiichiro Ueda 2, Norito Katoh 2, Masahiro Yamamoto 3, Kiyoshi Takeda 3, Shizuo Akira 4, and Shigeru Kinoshita 1

1 Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
2 Department of Dermatology,, Kyoto Prefectural University of Medicine, Kyoto, Japan
3 Laboratory of Immune Regulation, Department of Microbiology and Immunology, Graduate School of Medicine, Osaka University, Osaka, Japan
4 Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan

* To whom correspondence should be addressed. E-mail: mueta{at}ophth.kpu-m.ac.jp.


  Abstract

Purpose: I{kappa}B{zeta}-/- mice were reported to be affected by allergic dermatitis. To analyze the pathophysiological role of I{kappa}B{zeta} and to address the functional relevance of Th2-mediated immune responses in the development of ocular surface inflammation and dermatitis by I{kappa}B{zeta}-/- mice. Methods: We established Balb/c background I{kappa}B{zeta}-/- mice without individual differences, created I{kappa}B{zeta}/Stat6 double-knock-out (WKO) mice unable to produce Th2 cytokine, and performed microscopic-, histological-, and immunochemical studies. In I{kappa}B{zeta}-/- mice we examined the serum IgE levels by ELISA and used quantitative PCR to study the gene expression of IFN-{gamma}, IL4, IL10, TNF{alpha}, IL6, IL17{alpha}, and CCL11 in eyelid tissue. Results: I{kappa}B{zeta}-/- mice exhibited a severe inflammatory phenotype on the ocular surface and perioral skin. The inflammatory infiltrates in the perioral skin consisted primarily of CD4- and CD8-positive cells; in the conjunctiva we mainly detected CD4- and CD45R/B220-positive cells. In eyelid and perioral skin tissue the expression of IL-17{alpha} and of Th1 and Th2 cytokines, but not of CCL11, was augmented. I{kappa}B{zeta}-/- and I{kappa}B{zeta}-/+ mice did not differ significantly in their serum total IgE levels before- and 0-4- and 5-9 weeks after disease onset. I{kappa}B{zeta}/Stat6 WKO mice elicited the same or a little more severe inflammation than that of I{kappa}B{zeta}-/- mice. Conclusion: IgE and Stat6 are not responsible for the immune-pathological response leading to the development of ocular surface and perioral skin inflammation in I{kappa}B{zeta}-/- mice. I{kappa}B{zeta}-/- mice might be a suitable model for Stevens-Johnson syndrome (SJS), but not atopic dermatitis.

Key Words: I{kappa}B{zeta}-/- mice, ocular surface inflammation, Stevens-Johnson syndrome, dermatitis, Stat6






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2008 by the Association for Research in Vision and Ophthalmology