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From the A. Ray Irvine Ocular Pathology Laboratory, Doheny Eye Institute, and the Department of Ophthalmology, University of Southern California Keck School of Medicine, Los Angeles, California.
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METHODS. A group of Lewis rats underwent optic nerve axotomy with the application of N-4-(4-didecylaminostyryl)-N methylpyridinium iodide (4Di-10ASP) at the axotomy site. On days 3, 14, and 38 after axotomy, the rats were killed, the eyes were enucleated, and the retinas were stained for OX42. Another group of such axotomized rats were immunized with S-antigen peptide and were killed on days 7 through 12 after the injection with peptide. The enucleated eyes were stained for OX42 and examined by confocal microscope. After axotomy, bone marrow (Y
X) chimeric rats were injected with S-antigen peptide and were killed on days 10 and 12 after injection. The retinas were evaluated by PCR with Y-specific primers. Finally, a group of axotomized rats was injected with the S-antigen peptide and killed on days 6, 8, 9, and 10 after injection. Their enucleated eyes were examined for microglial expression of TNF
and for generation of peroxynitrite.
RESULTS. In the axotomized, non-EAU eyes, 4Di-10ASP–labeled ganglion cells were detectable on days 3 and 14, and 4Di-10ASP–containing OX42-positive cells (microglia) were found in the nerve fiber and other inner retinal layers on days 14 and 38. The S-antigen peptide–injected rats showed migration of the microglia (4Di-10ASP–positive and OX42-positive) to the photoreceptor cell layer on day 9, and these cells increased in number at this site on day 10. No macrophages (OX42-positive and 4Di-10ASP–negative) were present at this early stage of EAU, but such cells appeared in the retina on days 11 and 12. PCR of the chimeric EAU retinas showed an absence of the Y chromosome–amplified product on day 10, but the presence of this product was detected on day 12. The expression of TNF and generation of peroxynitrite were noted in the migrated microglia at the photoreceptor cell layer on days 9 and 10 of EAU.
CONCLUSIONS. In the early phase of EAU, the microglia migrate to the photoreceptor cell layer where they generate TNF and peroxynitrite. Such microglial migration and activation take place before infiltration of the macrophages. These findings indicate a novel pathogenic mechanism of EAU, in which retinal microglia may initiate retinitis with subsequent recruitment of circulation-derived phagocytes, leading to the amplification of uveoretinitis.
It has recently been proposed that microglia play an active proinflammatory role in the central nervous system (CNS) in several experimental and clinical diseases, including experimental autoimmune encephalomyelitis, cerebrovascular disease, Alzheimer’s disease, and multiple sclerosis.5 However, the identification of activated microglia in these pathologic conditions has been hampered by a lack of precise markers that can unambiguously distinguish the microglia from circulation-derived macrophages.
In a normal retina, microglia can be distinguished from macrophages primarily by morphologic features and perivascular distribution.4 However, when activated, as in pathologic conditions, microglia lose their highly branched morphology, acquire short processes, and become hypertrophied.6 7 8 These activated microglia share virtually all the morphologic features of infiltrating macrophages, including several cell surface markers, including ED1, -2, and -3; OX42; phosphotyrosine; and isolectin B4.7 Depending on the species of the animal and the state of microglial activation, some of these markers can probably distinguish microglia from macrophages.9 10 11 However, no such markers can distinguish activated microglia from macrophages.7 Retinal microglia are activated in EAU. Because no surface or other molecules can be used to specifically identify retinal microglia in EAU, a combination of multiple techniques is needed to distinguish the activated retinal microglia from macrophages.
In recent years, Thanos and Richter12 have reported a novel method in which the fluorescent dye N-4-(4-didecylaminostyryl)-N methylpyridinium iodide (4Di-10ASP; Molecular Probes, Eugene, OR) is used to detect retinal microglia after optic nerve axotomy. The dye initially appears in the retinal ganglion cells through retrograde axoplasmic transport. It is then taken up by the retinal microglia as a result of their phagocytosis of the dye-labeled ganglion cells. The retinal microglia containing this lipophilic dye remain labeled for up to 12 months.13 This type of long-term labeling may offer an opportunity for evaluating the role of retinal microglia in the initiation and perpetuation of EAU. However, the influx of circulation-derived monocyte-macrophages in EAU could phagocytose the degenerating dye-containing ganglion cells. Therefore this method per se may not unequivocally distinguish microglia from macrophages.
The male-to-female (YX) chimeric construction and the detection of Y chromosome-specific, donor-derived cells have been developed in recent years to track the outcome of transplanted cells in a mouse model. In such animals, the use of a Y chromosome-specific probe may allow identification of male bone marrow–derived cells. A rat cDNA probe specific for the Y chromosome has recently been reported.14 The use of bone marrow–chimeric rats with optic nerve axotomy, combined with the application of 4Di-10ASP may allow for the precise detection and localization of activated retinal microglia, and it may also facilitate differentiation of these native retinal cells from circulation-derived macrophages. Such differentiation is crucial for evaluating the role of microglia in EAU.
The purpose of this study was to devise methods that unequivocally identify the activated resident retinal microglia in EAU—especially, to distinguish them from the infiltrating macrophages—and to discern the role of resident microglia in the development of this intraocular inflammation. For this purpose, EAU was induced in chimeric (YX) rats after optic nerve axotomy. The mobility of microglia, as well as the arrival of hematogenous macrophages, was tracked sequentially, using confocal microscopy and polymerase chain reaction (PCR) to detect the Y chromosome. The pathogenic role of migrated microglia was assessed in vivo by detecting the generation of proinflammatory nitrogen species (peroxynitrite) and the expression of tumor necrosis factor- (TNF).
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In Vivo Labeling of Retinal Microglia
Six female Lewis rats were anesthetized, and a lateral canthotomy was performed on them, followed by a superior conjunctival peritomy. Dissection was performed posteriorly until the retrobulbar optic nerve was identified. A slit was created in the optic nerve sheath, parallel to the direction of the nerve fibers. Scissors were used to transect the optic nerve within the sheath, and 4Di-10ASP was packed at the cut surface of the optic nerve. The lateral canthotomy was then sutured. Axotomy was performed on right eyes only. To examine the retina in wholemount, three rats each were killed on postaxotomy days 3 and 14. The enucleated globes were fixed in phosphate-buffered 4% paraformaldehyde at 4°C for 4 hours. The globes were sectioned to discard the anterior segment and vitreous. The posterior segments were radially divided into four segments, and the retinas were separated from the choroids and incubated in 1% Triton X-100 in phosphate-buffered saline (PBS) for 1 hour and then washed in PBS. The retinas were incubated at 4°C overnight with mouse anti-rat monoclonal antibody OX42 (Serotec, Oxford, UK) 1:50 in 0.1% Triton X-100. Specimens were washed with PBS, then incubated with horse anti-mouse IgG conjugated with Texas red (1:100; Vector Laboratories, Burlingame, CA) at room temperature for 3 hours. The entire retina was flatmounted and the 4Di-10ASP and Texas red were visualized by confocal microscope (LSM510; Carl Zeiss, Oberkochen, Germany) with excitation and emission set at 488 and 530 nm, respectively, for 4Di-10ASP and 543 and 610 nm, respectively, for Texas red.
Migration of Retinal Microglia in EAU
Thirty-six rats were axotomized as described earlier and separated into two groups of 18 rats each for one control group and one EAU group. Of the 18 control rats, 6 rats were killed on each of postaxotomy days 3, 14, and 38. On postaxotomy day 28, the second group of 18 rats was immunized with human S-antigen peptide (DTNLASSTIIKEGIDRTVLG) to induce EAU, as described previously.1 On days 7 through 12 after the peptide injection, three animals were killed each day. Frozen sections (14 µm) of the globes were prepared from these rats and from nonimmunized rats and stained with OX42. The procedure used for OX42 staining was the same as that described for the wholemounted retina, except that the incubation with Triton X-100 was omitted and a 1:100 dilution was used for the primary antibody. The confocal microscope was used to visualize the dye and OX42 staining as described. Adjacent sections of the retina were obtained, stained with toluidine blue, and examined by compound microscope for histologic changes caused by EAU.
Two areas of each retina, superior and inferior, approximately 1 mm from the optic disc were selected for the cell count in the photoreceptor cell layer. Cells displaying positive staining for OX42 or exhibiting staining for both 4Di-10ASP and OX42 were counted at a magnification of 500x. Cells that were positive for both OX42 and 4Di-104SP were counted as microglia. Those that were OX42-positive and 4Di-10ASP–negative were counted as macrophages.
Detection of Y Chromosome–Containing Cells by PCR
A 104-bp probe was generated from the genomic DNA of male rats using primers selected to the murine Sry gene, the sex-determining region of the Y chromosome.14 15 Briefly, genomic DNA from male rat spleen was subjected to PCR amplification with the following pair of primers: 5'-CATCGAAGGGTTAAAGTGCCA-3' (5' primer) and 5'-ATAGTGTGTAGGTTGTTGTCC-3' (3' primer).14 The amplified product (Y-104 bp) was confirmed by agarose gel electrophoresis, with a 100-bp DNA ladder used as a molecular weight marker. Cloning was performed according to the method described by Sambrook et al.16 Briefly, PCR-amplified Y-104-bp fragments were directly ligated to the T overhangs of vectors (pGEM; Promega, Madison, WI). The DH52 CaCl2–competent cells were transfected with circular plasmids. The recombinants were screened using the blue–white selection procedure. Three white colonies were randomly picked up and cultured. Plasmid DNA was then isolated and subjected to restriction enzyme digestion and agarose electrophoresis, with a 100-bp DNA ladder used as a molecular weight standard. The inserts were sequenced according to the method described by Sanger et al.17 To determine the specificity and sensitivity of the Y-104-bp fragments for male DNA, male-female splenic DNA mixtures were prepared in the ratio ranging from 1:1000 to 1:100 of male-to-female DNA (in nanograms). A similar experiment for assessing the limit of PCR detection was performed, with male-female spleen cells also mixed in the range of 1:10,000 to 1:100. These mixtures were amplified for 35 cycles using primers for a Y-104-bp fragment.
Construction of Chimeric Rats and Determination of Stability of the Retinal Microglia Compartment
Thirty-two chimeric (YX) rats were constructed by using sterile techniques. The bone marrow removed from the tibia, femur, and humerus of the male donor rats was passed through a density gradient medium (Ficoll-Paque Plus; Amersham Pharmacia Biotech, Piscataway, NJ) to remove red blood cells and cell debris. The cells were washed with RPMI 1640 (Gibco BRL, Rockville, MD) supplemented with 10% fetal bovine serum. Viability of the nucleated bone marrow cells was greater than 95%, as determined by trypan blue. The female rats were irradiated with 1000 rads by a cesium-135 gamma irradiator (Gamma Cell-40; Atomic Energy of Canada, Ltd., Ottawa, Ontario Canada) and then received intracardiac injections of 5 x 107 bone marrow cells in 1.0 mL Hanks’ balanced salt solution.
After establishment of chimerism (60 days from bone marrow transplantation), four of the animals were killed, and DNA extracted from their spleens was submitted to PCR to detect a Y-chromosome–specific 104-bp fragment. Twenty-one of the chimeric animals underwent right optic nerve axotomy as described earlier, and seven animals from this group were killed on each of postaxotomy days 7, 14, and 28 (corresponding to days 67, 74, and 88 after bone marrow transplantation). The animals were perfused with normal saline to remove any intravascular circulating cells. Retinal DNA was extracted (Easy-DNA kit; Invitrogen, Carlsbad, CA), and the pellet was dissolved in 50 µL Tris EDTA and quantified. Similarly, DNA samples from the spleens and lungs were obtained and quantified. Polymerase chain reaction was performed with the primers and 35 cycles as described earlier.
Resident Retinal Microglia in the Chimera
This experiment was designed to test whether total body irradiation and bone marrow transplantation may by themselves alter the number of retinal microglia. Sixty days after the construction of chimeras, three chimeric rats and three nonchimeric rats underwent right optic nerve axotomy with application of 4Di-10ASP, as described earlier. Fourteen days after the axotomy, the animals were killed, and the enucleated eyes were processed for flat preparation and mounted for confocal visualization. Five retinal fields adjacent to the optic disc were randomly selected, and the number of 4Di-10ASP–positive cells in 0.09 mm2 was counted.
Macrophages in EAU Chimeric Rats
Two months after the chimeric construction, EAU was induced in four chimeric (YX) rats by immunizing with S-antigen peptide as described earlier. Two animals each were killed on postimmunization days 10 and 12. Histopathologic examination was performed on half of the retina and the whole anterior segment from each eye. DNA was extracted from the remaining retina (Easy-DNA kit; Invitrogen) and subjected to Y chromosome detection by PCR amplification, as described earlier.
Generation of TNF and Peroxynitrite by Retinal Microglia in EAU
Twelve Lewis rats were axotomized and 4Di-10ASP was deposited on the cut stump of the optic nerve, as described earlier. At postaxotomy day 28, all rats were immunized with S-antigen peptide, also described earlier. Three animals were killed on each of days 6, 8, 9, and 10 after S-antigen injection. The enucleated globes were divided into two half portions, and each half was tested for either TNF or peroxynitrite (ONOO-).18 19 For TNF- localization, 14-µm cryostat sections of the retina were fixed with 4% paraformaldehyde at room temperature for 10 minutes and then exposed to primary antibody and goat anti-rat TNF (1:100, R&D Systems, Minneapolis, MN) and subsequently to a secondary antibody, rhodamine-conjugated anti-goat IgG (1:100; Vector Laboratories). The immunostained sections were examined by confocal microscope as described earlier; except, the 543-nm laser beam for the excitation of rhodamine fluorophore was switched off for visualization of 4Di-10ASP, and the 488-nm excitation beam was switched off for visualization of rhodamine, to eliminate any possible cross talk between the two fluorophores.
For detection of ONOO-, the retinas were exposed to dihydrorhodamine 123 (Molecular Probes) in medium supplemented with B27 (Gibco BRL).19 The dihydrorhodamine was dissolved in dimethyl sulfoxide (Sigma, St. Louis, MO) to a concentration of 5 mM, and this stock solution was further diluted to a 50-µM working solution with Dulbecco’s modified Eagle’s medium. Retinas were incubated in working solution for 3 hours before 14-µm frozen sections were obtained. Visualization was performed with the confocal scanning microscope, as described earlier.
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Construction of Chimeric Rats and Determination of the Stability of the Retinal Microglia Compartment
PCR analysis of DNA extracted from the spleens of the three chimeric animals 2 months after (YX) bone marrow transplantation revealed the presence of a Y-chromosome–specific 104-bp fragment, indicating the successful construction of chimeric animals. Neither the retina of the right axotomized eyes nor the retina of the left nonaxotomized eyes of the chimeric animals revealed the presence of Y chromosome, irrespective of whether the rats were killed on postaxotomy day 7, 14, or 28. These results indicate that the retinal microglial compartment is stable and devoid of any repopulation by peripheral blood-derived cells, even 88 days after bone marrow transplantation. At this stage, DNA obtained from the spleen and lungs of these animals showed the 104-bp amplified product.
Resident Retinal Microglia in the Chimera
In the control rat retinas, there were 46.7 ± 9.4 microglia/0.09 mm2, whereas in the chimeric retinas, there were 53.2 ± 13.0 microglia/0.09 mm2. Although there appeared to be a trend toward an increased number of microglia in the chimeric animal retinas, the difference between the chimeric and control animals was not significant (P = 0.07; Fig. 6 ).
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X) animals injected with the S-antigen peptide, one of the four eyes showed the PCR amplification product of the Y chromosome on day 10. The same eye also exhibited histologic evidence of inflammation, including infiltration of polymorphonuclear neutrophils (PMNs) and mononuclear cells and disrupted retinal architecture. The other three 104-bp negative eyes showed no such evidence of inflammatory cell infiltration, and the retinal architecture was intact. In contrast, on day 12, three of the four eyes were positive for Y chromosome (Fig. 7) , and there was histologic evidence of retinitis, characterized by infiltration of monocytic cells and disrupted retinal architecture.
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and ONOO- by the Migrated Retinal Microglia in EAU in the retina after injection of S-antigen peptide, rats killed on days 6 and 8 were all negative for TNF staining, but the rats killed on days 9 and 10 showed positive staining for TNF within the 4Di-10ASP–positive cells (microglia). The dual-labeled (4Di-10ASP–positive and TNF-rhodamine–positive) cells were localized predominantly in the photoreceptor layer, and the TNF-rhodamine–positive cells matched the 4Di-10ASP–labeled microglia that migrated to the outer retina (Fig. 8) . There was also a small population of dual-labeled cells in the inner retina.
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The PCR (for the Y chromosome) of the retinas from axotomized chimeric animals showed the absence of the Y-chromosome–amplified product, indicating that the 4Di-10ASP–positive and OX42-positive cells are indeed microglia. Thus, this complex experimental approach could discern microglia from circulation-derived macrophages (Y-chromosome–possessing cells). Moreover, EAU induction in such animals showed that the macrophages (OX42-positive, 4Di-10ASP–negative cells) appeared in the retina when PCR for the Y chromosome became positive (Figs. 3 4 7) , indicating that the macrophages did not phagocytose the dye-containing cellular debris. This allowed us to use a morphologic approach to positively distinguish the microglia from macrophages. The microglia were positive for both 4Di-10AP and OX42, and the macrophages were positive for OX42, but negative for 4Di-10ASP.
In the present study, we took advantage of the initial period at days 28 to 30 postaxotomy, when microglia became stabilized after phagocytosis of the ganglion cells.20 21 After the induction of EAU in the axotomized rats, the retinal microglia (dual stain-positive for OX42 and 4Di-10ASP) migrated to the outer retina–photoreceptor cell layer at the early phase of EAU (days 9 and 10 after immunization). When the disease reached the amplification phase (days 11 and 12 after immunization), several of the OX42-positive cells were negative for 4Di-10ASP (macrophages), whereas others that were positive for both 4Di-10-ASP and OX42 (microglia) were localized to the photoreceptor layer. The PCR of the retina for the Y chromosome became positive at this amplification phase. These findings clearly show that microglia migrate to the outer retina during the initial phase of uveitis, and macrophages subsequently appear during the amplification phase. These results confirm that the cells that initially appear at the site of the target retinal antigen are microglia and suggest that they may play a role in the recruitment of bone marrow–derived cells at the site of the target antigen. Moreover, we found that the migrated microglia release two proinflammatory mediators, TNF and ONOO- (Figs. 8 9) , in the photoreceptor cell layer before the arrival of macrophages. The release of these mediators suggests that the microglia participate effectively in the initiation of the inflammatory effector process that leads to photoreceptor degeneration.
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The retinal microglia have been shown to be highly migratory, especially in response to retinal insults such as optic nerve transection, focal laser lesions, photoreceptor degeneration, and IL-1-induced inflammation.9 25 26 27 More recently, exposure to light has been found to induce migration of mouse retinal microglia into the subretinal space, with the uncleared aging photoreceptors acting as the chemoattractant that recruits the microglia from the inner nuclear layer to the subretinal space.28 However, the exact mechanism underlying the activation and subsequent migration of microglia is still largely unknown.
Although microglia have low mitotic activity,29 for chimeric animals to be useful in delineating retinal microglia from the blood-derived monocytes, the stability of the microglial compartment must be maintained during the entire period of the experiment and beyond. It is also important that any subsequent experimental intervention, such as transection of the optic nerve, not change this stability. On postaxotomy day 14 in the chimeric rats (day 74 after bone marrow transplantation), we found that the retinal microglial cell counts in the chimeric and nonchimeric rats were not the same. However, the difference between the two groups was small and not significant (P = 0.07). Using PCR detection, repopulation engraftment was found to be rapid in hematopoietic tissues such as the spleen and, to a lesser extent, the lung. This repopulation was absent in the retina and the brain parenchyma as much as 30 weeks after the transplantation. These results are consistent with recently a published kinetic study of engraftment in the mouse after bone marrow transplantation, in which 89% of donor cells were detected in the spleen.30 After 1 year, 61% were detected in the lung and 30% in perivascular and leptomeningeal sites, but none were detected in the parenchyma of the brain.30 This recent study dealt with bone marrow transplantation in syngeneic mice. In our experiments, we transplanted marrow from male Lewis rats to female Lewis rats. When Lewis rats and (DAxLewis) F1 hybrids are used as bone marrow donors, the meningeal and perivascular monocytes are slowly replaced by hematogenous cells under normal conditions, whereas resident microglia represent a stable cell pool that is only exceptionally replaced by hematogenous cells in adult animals.31 These results contrast with a higher rate of repopulation encountered in xenogenic transplantation, in which graft-versus-host disease is evident.32 Our experimental approach clearly shows that the retinal microglial compartment is a stable one, even at 30 weeks after transplantation, indicating that there is a stable microglial compartment at the time of development of EAU without engraftment of blood-derived monocytes in the retina.
In vitro studies on microglia show that these cells produce superoxide, nitric oxide, and TNF.30 31 32 33 34 35 The relationship of these cytotoxic agents to neuronal death has been verified by an experiment in which inhibition of microglial invasion into the photoreceptor cell layer promotes the survival of photoreceptor cells in the dystrophic retina.36 In our EAU animals, the microglia were recruited to the photoreceptor layer very early in the disease state, particularly before infiltration of hematogenous macrophages and other phagocytes. Moreover, our histochemical observations showed that microglia that are localized at the photoreceptors generate TNF (Fig. 8) and ONOO- (Fig. 9) . ONOO- is the most potent biological oxidant capable of oxidizing cellular components.3 35 In CNS inflammation, microglia continue to express TNF as part of the maintenance and amplification of the inflammatory cascade. In vitro data suggest that TNF can cause the release of other cytokines, such as IL-1ß and IFN
, from reactive astrocytes.22 Unlike the production of oxidants, production of TNF is not associated with phagocytosis.37 Therefore, the production of TNF by the migrated microglia before the arrival of blood-born phagocytes in the EAU indicates that microglia have a proinflammatory and pathogenic role, rather than the mere phagocytic function of eliminating the damaged photoreceptors.
In summary, the precise in vivo identification of activated retinal microglia and their migration into the photoreceptor layer before the infiltration of hematogenous cells in the initial phase of uveoretinitis reveals a novel pathogenic mechanism by which the retinal inflammation may begin in autoimmune uveoretinitis and by which subsequent retinal degeneration may take place in the inflammatory process. These findings further expand current belief about the role of microglia, in which only their scavenger role, rather than their pathogenic role, in retinal diseases has been emphasized. Because retinal damage in uveitis is caused by the amplification process, a better understanding of the initial inflammatory process may lead to the development of novel, locally acting, anti-inflammatory agents directed to the microglia. Such a specific treatment would be ideal, in that it would avoid the present day systemic treatment with its associated complications.
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Submitted for publication February 26, 2002; revised June 28, 2002; accepted July 16, 2002.
Commercial relationships policy: N.
The publication costs of this article were defrayed in part by page charge payment. This article must therefore be marked "advertisement" in accordance with 18 U.S.C. 
1734 solely to indicate this fact.
Corresponding author: Narsing A. Rao, Doheny Eye Institute, 1450 San Pablo Street, DVRC-211, Los Angeles, CA 90033; nrao{at}usc.edu.
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, macrophages.
