Supplementary Videos

Matthew R. Ritter, Edith Aguilar, Eyal Banin, Lea Scheppke, Hannele Uusitalo-Jarvinen, and Martin Friedlander

Files in this Data Supplement:

• Supplementary Video 1 - 2.93 MB (.avi)
  Ocular vasculature of an 8-day-old mouse imaged in vivo. Vasculature was visualized by intravenous injection of fluorescent dye and use of a scanning laser confocal microscope. Visible in this image are (1) vessels of the iris, (2) the pupillary membrane, which traverses the iris and supplies the anterior aspect of the lens during development, (3) the tunica vasculosa lentis (TVL), which is in close opposition to the posterior lens surface, and (4) the vasa hyaloidia propria which lies just posterior to the TVL. The lens is found within the large avascular (black) region between the pupillary membrane and the TVL. The retinal vessels are only faintly represented in this image. Supplementary Video 2 shows these structures following an additional 8 days of developmental regression.

• Supplementary Video 2 - 2.79 MB (.avi)
  Ocular vasculature of a 16-day-old mouse imaged in vivo. By 2 weeks of age, the pupillary membrane has fully regressed, as evidenced by the lack of vessels within the area bound by the iris (pupil). The vasa hyaloidia propria has also regressed by this time, and the tunica vasculosa lentis is undergoing pruning. The retina is represented in some detail in this image with even small caliber capillaries visible.

• Supplementary Video 3 - 2.49 MB (.avi)
  Hyaloidal vasculature of an 8-day-old mouse imaged in vivo. This image was isolated from Supplementary Video 1 to show only the vessels of the tunica vasculosa lentis and vasa hyaloidia propria.

• Supplementary Video 4 - 2.68 MB (.avi)
  Hyaloidal vasculature of a 16-day-old mouse imaged in vivo. This image was isolated from Supplementary Video 2 to show only the vessels of the hyaloid. Note the marked pruning of the tunica vasculosa lentis and the absence of the vasa hyaloidia propria when compared to Supplementary Video 3.

• Supplementary Video 5 - 2.58 MB (.avi)
  Color depth-coded image of the ocular vasculature in a P16 mouse. To aid in distinguishing vascular structures in the eye, different regions were color coded during image processing according to their depth. Red = iris vessels, blue = hyaloidal vessels, green = retinal vasculature. The same data used to create the grayscale rendering in Supplementary Video 2 was used here.

• Supplementary Video 6 - 4.04 MB (.avi)
  Iris and retinal vasculature of a 32-day-old mouse imaged in vivo. This image shows a peripheral region of the retina and shows major retinal vessels, as well as capillaries, in detail. A single remnant of the hyaloid vasculature is seen just anterior to the retinal vessels.

• Supplementary Video 7 - 5.29 MB (.avi)
  In vivo imaging of a central retinal vein occlusion model. A laser-induced occlusion was made 3 days prior to imaging. Slow filling of the occluded retinal vein (lower center) can be observed via two venules (one on each side of the occluded vein). The occlusion is clearly visible toward the end of the video as a discontinuous region in the slowly filled vein. Image motion was caused by movement of the mouse during image capture. Duration of image capture is 20 min.

• Supplementary Video 8 - 3.54 MB (.avi)
  In vivo imaging of ocular pathology in a mouse model of oxygen-induced retinopathy at P14. This mouse was subjected to a course of hyperoxia using an established model and imaged at postnatal day 14 (2 days after return to normal oxygen conditions). The major features observed are (1) hyaloid tortuosity, (2) central vaso-obliteration of retinal capillaries, (3) tortuosity of retinal vessels, and (4) venous dilation.

• Supplementary Video 9 - 3.29 MB (.avi)
  In vivo imaging of ocular pathology in a mouse model of oxygen-induced retinopathy at P18. These images were collected at postnatal day 18, 6 days after return to normal oxygen conditions and during the neovascular phase of this model. In addition to the hyaloidal and retinal vascular tortuosity that persists, evidence for pre-retinal neovascularization is indicated by the enlarged regions of retinal vessels. These are likely sites of dye extravasation from the neovessels which have been reported previously to be prone to leak fluid. The vasculature of the optic disc area is often affected in this model, and evidence of abnormal vascularization in this area is seen here.